Optimization of the Green Chemistry-like Extraction of Phenolic Compounds from Grape (Vitis labrusca L.) and Blackberry (Rubus fruticosus L.) Seeds with Concomitant Biological and Antioxidant Activity Assessments

The objective of this work was to determine the phenolic composition, chemical and cellular antioxidant activity, cytotoxicity in human cells, peroxidative inhibition defatted fraction grape (Vitis labrusca) blackberry (Rubus fruticosus) seeds. Soxhlet extraction (Sox) used extract fat obtain degreased material. A statistical optimization study developed maximize bioactive compounds activity from Simultaneous applied with a combination 35.9 min solid-to-solvent ratio 1 g seed 61.28 mL an extracting solvent (60% ethanol) 62.1 64.1 ethanol). In cell viability assay, HepG2 cancer cells seemed more sensitive extracts, while Ea.hy926 hybrid showed resistance their effects. general, extracts presented low/no cytotoxicity, exhibited protective effect against H2O2-induced ROS production, demonstrated on erythrocytes when subjected hypotonic isotonic conditions not presenting hemolytic behavior (5.0 10.0 μg GAE/mL). Thus, results provided broad assessment bioactivity obtained using simple low-cost process by employing non-toxic solvents potential be technological applications.